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Proliferation of human melanoma cells is under tight control of protein kinase C alpha.
Krasagakis, Konstantin; Lindschau, Carsten; Fimmel, Sabine; Eberle, Jürgen; Quass, Petra; Haller, Hermann; Orfanos, Constantin E.
Affiliation
  • Krasagakis K; Department of Dermatology, University Medical Center Benjamin Franklin, Free University of Berlin, Berlin, Germany. krasagak@med.uoc.gr
J Cell Physiol ; 199(3): 381-7, 2004 Jun.
Article in En | MEDLINE | ID: mdl-15095285
Exponential proliferation of human melanoma cells has been associated with low levels of protein kinase C (PKC)-alpha. The aim of the present study was to investigate the functional relationship between PKC-alpha and melanoma cell proliferation. Treatment of human melanoma cells with the selective PKC inhibitor Ro-31-8220 resulted in a significant increase of cell proliferation as measured by (3)H-thymidine incorporation and a fluorometric microassay. In addition, phosphorothioate antisense-oligodeoxynucleotides (ODNs) to PKC-alpha enhanced DNA-synthesis of human melanoma cells. Furthermore, microinjection and transient transfection of melanoma cells with PKC-alpha decreased their proliferation, as shown by the reduction of nuclear staining with the proliferation marker Ki-67. The presented data demonstrate a cause-effect relationship between PKC-alpha and melanoma cell growth, whereby PKC-alpha reversely influences the rate of cell proliferation.
Subject(s)
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Collection: 01-internacional Database: MEDLINE Main subject: Protein Kinase C / Melanoma Limits: Humans Language: En Journal: J Cell Physiol Year: 2004 Document type: Article Affiliation country: Country of publication:
Search on Google
Collection: 01-internacional Database: MEDLINE Main subject: Protein Kinase C / Melanoma Limits: Humans Language: En Journal: J Cell Physiol Year: 2004 Document type: Article Affiliation country: Country of publication: