Proliferation of human melanoma cells is under tight control of protein kinase C alpha.
J Cell Physiol
; 199(3): 381-7, 2004 Jun.
Article
in En
| MEDLINE
| ID: mdl-15095285
Exponential proliferation of human melanoma cells has been associated with low levels of protein kinase C (PKC)-alpha. The aim of the present study was to investigate the functional relationship between PKC-alpha and melanoma cell proliferation. Treatment of human melanoma cells with the selective PKC inhibitor Ro-31-8220 resulted in a significant increase of cell proliferation as measured by (3)H-thymidine incorporation and a fluorometric microassay. In addition, phosphorothioate antisense-oligodeoxynucleotides (ODNs) to PKC-alpha enhanced DNA-synthesis of human melanoma cells. Furthermore, microinjection and transient transfection of melanoma cells with PKC-alpha decreased their proliferation, as shown by the reduction of nuclear staining with the proliferation marker Ki-67. The presented data demonstrate a cause-effect relationship between PKC-alpha and melanoma cell growth, whereby PKC-alpha reversely influences the rate of cell proliferation.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Protein Kinase C
/
Melanoma
Limits:
Humans
Language:
En
Journal:
J Cell Physiol
Year:
2004
Document type:
Article
Affiliation country:
Country of publication: